Liquid nitrogen biological container is abbreviated as liquid nitrogen tank, which is widely used in animal husbandry, medical beauty, food, electronics, machinery, laboratory, scientific research and other fields. With the continuous development of society, the application of liquid nitrogen tanks has become more and more extensive. So, how should we choose a liquid nitrogen tank?
I. Determine the purpose
According to the purpose, liquid nitrogen tanks can generally be divided into liquid nitrogen storage tanks and liquid nitrogen transportation tanks.
1.The liquid nitrogen storage tank is mainly used to store liquid nitrogen for later low-temperature experiments or to store samples that require low-temperature preservation, such as cells and tissues (used for indoor static storage of liquid nitrogen, not suitable for long-distance transportation under working conditions).
2. The liquid nitrogen transportation tank is used to transport low-temperature sample. In order to meet the transportation conditions, a special shock-proof design is made. In addition to static storage, it can also be used for transportation when filled with liquid nitrogen, but severe collisions and vibrations should also be avoided.
II. Determine the amount, diameter and volume of the sample to be frozen
1. The amount of frozen specimens will determine the size of the liquid nitrogen tank. If there are few specimens, choose a small-volume liquid nitrogen tank. If the liquid nitrogen needs to be stored for a longer time, we can choose a 30-liter or 35-liter 50mm liquid nitrogen tank with a diameter of 50 mm for storage. The static storage days of liquid nitrogen are more than half a year.
2. If the sample volume is large, we should choose a larger liquid nitrogen tank. If we buy a small volume liquid nitrogen tank for freezing, the sample will take up its space inside, and it can only contain a little liquid nitrogen. This will reduce the storage days of liquid nitrogen, which may cause necrosis of frozen specimens due to lack of liquid nitrogen.
3. The size of the specimen diameter is also critical for the selection of liquid nitrogen tanks. Generally, the diameter of the liquid nitrogen tank should be larger than the diameter of the specimen to be frozen. For example, if the specimen diameter is 90mm, but we purchase a liquid nitrogen tank with a diameter of 80mm, the specimen will not fit in, and our needs cannot be met.
There are about two kinds of buckets inside the liquid nitrogen tank. One is a round bucket, which can be used to place larger tissue samples or large-capacity cryotubes. The other is an lift-pull square bucket, which can be placed in a standard 2ml cryotube to store cells and other products.
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