Liquid nitrogen biological containers are abbreviated as liquid nitrogen tanks. According to the GB/T5458-1997 national recommended standards, liquid nitrogen tanks are divided into liquid nitrogen storage tanks, liquid nitrogen transport tanks, and self-pressurized liquid nitrogen tanks according to their purpose, and their efficacy is different.
 
Liquid nitrogen tanks are widely used in the preservation of semen of cattle and sheep and the active preservation of biological samples, with a wide range of applications. With the continuous development and improvement of science and technology, the cryogenic treatment of metal materials by liquid nitrogen can change the metallurgical structure of metals and improve the strength and hardness of metal materials to improve  the wear resistance of equipment.
 
The liquid nitrogen tank is safe without liquid nitrogen. However, if it is filled with liquid nitrogen, you should pay attention to safety issues, and it mainly includes the following aspects:
1. Do not touch liquid nitrogen. If it is in contact with the skin for too long, it will burn the skin, and the contact with eyes will seriously affect the vision.
2. Use tweezers to pick and place semen. If the skin is in direct contact with the metal bucket, it will be stuck and torn.
3. Insert the object slowly to prevent the liquid nitrogen from spilling out. Because liquid nitrogen will boil when an object is inserted, and it may splash out of the tank, so the action should also be slow when filling nitrogen.
4. Only use the stopper of liquid nitrogen tank to prevent the evaporation of liquid nitrogen. If other plugs are used, the pressure of the liquid nitrogen tank may increase, leading to  explosion or liquid nitrogen loss.
 
When using a low-temperature liquid nitrogen tank, it needs to be used in accordance with the normal operating method. The storage of the liquid nitrogen tank needs to be in an air-circulating environment, because the liquid nitrogen will stay indoors after evaporation. Once the liquid nitrogen is too much, it will cause the shortage of oxygen. Therefore, when using the liquid nitrogen tank, we need to handle it with care to avoid collision with other objects, because the liquid nitrogen tank is in a vacuum state, and when the inner and outer tanks are exposed to atmospheric pressure, they need to go through three times the strength of their own weight. So be careful about falling or collision, and it is strictly prohibited to dump the liquid nitrogen in the liquid nitrogen tank, and pay attention to the safety of personnel.